Application

PCR qPCR Southern Blot Methylation-sensitive PCR CpG array Restriction enzyme digestions Viral Detection Bacterial Detection Microarray NGS

General description

Sigma’;s GenElute^™ Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit provides a fast, reliable and simple procedures for isolating cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 4 mL. Purification is based on spin column chromatography that uses Sigma’;s proprietary resin separation matrix. The kits are designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS. Isolate all sizes of circulating DNA from plasma and serum samples Isolate viral and bacterial DNA Versatile plasma and serum input volumes (1 mL – 4 mL) Concentrate circulating DNA into a flexible elution volume ranging from (50 µL - 100 µL) Isolate inhibitor-free cell-free circulating DNA Purify high-quality DNA in 40-45 minutes Compatible with Streck Cell-Free DNA BCT Tubes

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Unit Definition

Preps